For various type of Animal tissue samples, Phenol based solution type RNA Extraction Kit – Tissue RNA PrepMate™
Tissue RNA
PrepMate™ uses a modified guanidium salt-lysis method to optimally extract total RNA from tissue. The amount of extracted total RNA can differ according to the tissue or cell type, so the starting amount should be adjusted to your needs. The extracted viral RNA can be used as a template of the RT-PCR or quantitative real time RT-PCR and cDNA synthesis, cDNA library construction, Microarray.
Features and Benefits
Advantage
It is extracted through the following steps; lysis, phenol:chloroform (5:1), and isopropanol precipitation. Genomic DNA contamination is minimal, even without DNase treatment. Total RNA is extracted quickly with high purity (OD260/280 > 1.8).
Experiment Time
1 hr (in case of tissue)
Sample source
Tissue, Serum, Cultured cell, Leucocyte
Application
Northern analysis, dot blot hybridization, poly (A) selection, RNase protection assay, RT-PCR and Cloning.
Contents
Components |
Quantity |
Note |
Lysis Buffer |
1 ea |
100 ml |
Phenol/ Chloroform (5:1) |
1 ea |
100 ml |
RNA Hydration Buffer |
1 ea |
10 ml |
Manual |
1 ea |
|
Applications
Northern /dot blot analysis, polyA+ selection, RNase protection assay, RT-PCR, real-time RT-PCR, cloning
Procedure
Manual
• Tissue RNA PrepMate™
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
Cat.No.
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Product Description
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Price
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Add to Cart
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K-3080
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Tissue RNA PrepMate™ 100 reactions
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Inquire
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