Oligo Synthesis Service Order Oligos

Individual needs, high throughput or bulk quantities; Bioneer has your oligo bases covered.

Bioneer, founded in 1992, is one of the world’s leading suppliers of synthetic oligonucleotides (DNA and RNA).
In-house production of raw materials (phosphoramidites, reagents & solvents) combined with automated oligo synthesis and purification systems, results in superior quality oligonucleotides at a great price.
Since Bioneer manufactures all of the components that go into our oligonucleotides, we manage quality control every step of the way – ensuring that you receive only the highest quality product.
Every Bioneer oligonucleotide is purified free of charge utilising our unique Bio-RP cartridge purification technology. Bio-RP removes many impurities and synthesis failure products that are still present in an oligo after desalting*.
These impurities contribute to the OD measurement of competitor oligonucleotides that are merely desalted – and artificially inflate yield (see below).
Bioneer’s standard Bio-RP cartridge purification is near HPLC quality with only full-length product resulting. 
We are so confident with our oligonucleotide quality that we are the only company to offer a 100% guarantee that they will work for your PCR or qPCR application!
With high throughput oligo synthesis facilities around the world, Bioneer’s daily capacity is unsurpassed.
Bioneer is unrivalled in its ability to address the needs of customers requiring a few oligonucleotides on a regular basis and those customers that require very large numbers of oligonucleotides on a less frequent basis. We will also respond to your special customisation needs – personally.
 

Features and Benefits

• Free Bio-RP purification: Near HPLC purity at a standard oligo price
• Quick turnaround time: < 3.5 business days from order to bench in most cases
• Broad range of modifications available: If you don’t see it, just ask, we can probably do it
• Competitive pricing: Great value for your research dollar

Why is Bio-RP purification better than desalting?

Most oligos sold are merely deprotected/desalted. The process of deprotection/desalting only partially purifies the oligo and leaves behind many impurities* and failure products (truncated oligos that will not amplify and that may interfere with certain applications). These can lead to artificially inflated OD readings that increase with oligo length.
Bio-RP purification removes these contaminants and failure products, resulting in an oligo that demonstrates near HPLC purity. The advantage to you is that our product contains only quality oligos minus impurities that may inhibit some PCR reactions and that potentially skew qPCR reactions.
We deliver the same amount of active oligo as our competitors; they just have more “other” artificially inflating their OD.
To demonstrate this, plates of Oligos of various length were tested for concentration in 2 steps:
1) following a deprotection/desalting step only, and 2) following Bio-RP purification. (Fig. 1)

The results of these tests show that the amount of failed sequences and impurities* increases with length (as expected), but the failure product is not removed by deprotection/desalting alone. This results in inflated OD readings for oligos depending on oligo length.
(Table 1)

Figure 1. Plates of Oligos of various length were tested for concentration following desalting and then by Bio-RP cartridge purification.
Note inflated OD readings when deprotection/desalting alone is used.
 

Table 1. Average OD inflation for oligos of various length when protection/desalting alone is employed v. Bio-RP purified product.
Note for longer oligos; up to 77% of product does not consist of functional oligo.

*Impurities in desalted oligos may include: Acetonitrile, Pyridine, Iodine,
Ethylthiotetrazole, Dichloromethane, Acetic acid, Acrylonitrile, Benzamide and Isobutyramide.

PAGE Oligo Purification

PAGE Oligo purification is recommended for Oligos between 50-100 bases. This results in yields with purity levels exceeding 95%. Subsequently yield obtained is lower than when other purification techniques are applied.

HPLC Oligo Purification

HPLC purification can be used for both modified and unmodified oligos up to 50 bases in length. Our HPLC oligo purification always begins with a trityl-on purification, and is often followed by a second full gradient trityl-off HPLC. In some cases, the initial trityl-on purification is entirely sufficient and a cartridge is utilized in the subsequent trityl-off oligo processing. This RP-HPLC purification process can result in yields of highly pure material, especially when the oligonucleotide is not complex. When synthesizing compounds that require post-synthesis modification such as dye labeling, we will apply an additional RP-HPLC oligo purification.

AccuOligo® is Bioneer's patented oligo technology.

AccuOligo® prevents oligo dislodgment and potential loss during packing, shipping and opening through the addition of a patented adhesive to the tube during the production process. Even 96-deep well plate format orders have AccuOligo® technology applied, preventing cross-contamination from occurring during use.
(Patent application. Reg. Number 10-0777249 )

The adhesive does not affect PCR, Sequencing, restriction digests or other experimental methods
Prevention of dislodgment and loss of oligos during production, packaging and shipping
Prevents loss due to oligo "flaking" and tube cap attachment loss
Prevents cross-contamination when using plate formats
Quantified oligos made to order

Bioneer's in-house developed Bio-RP purification system.

Bioneer's R&D team has developed an RP resin for purification, as a superior alternative to standard desalting/deprotection as offered by our competitors. This delivers near-HPLC quality and is provided Free of Charge.
 

Contamination prevention through clean room production.

All oligos manufactured at Bioneer are produced within clean room facilities, ensuring that we provide nuclease/nucleic acid-free product to our customers.
 

Molecular weight-level quality control with accurate MALDI- TOF QC.

Every oligo produced is QC'd using MALDI-TOF and 96-well CE Q.C systems.
A comprehensive QC report is shipped with each oligo to ensure confidence and to ensure that our customers receive only the highest quality products for use in their research.

Ordering Information

Standard Custom
High Throughput
Modified
Dual Labeled Probes
Extendamers™
Large Scale