AccuPower® Taq PCR Premix

The AccuPower® Taq PCR PreMix is a convenient lyophilized PCR master mix containing Taq DNA polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer and is aliquoted in 8-strip PCR tubes. The premix retains its activity for over a month at room temperature and is stable for two years in -20°C freezer. AccuPower® Taq PCR PreMix is available with or without tracking dye, depending on your application. If purchased with tracking dye, reactions can be loaded on agarose gels without adding loading buffer.

 Features and Benefits

• Flexible: Taq provides accurate amplification of standard and highly suitable for all PCR applications.
•  Easy to use: All reaction components required for PCR, including thermostable DNA polymerase and dNTPs are contained within each tube and in a lyophilized "PreMix" form. 
•  Reproducibility: Bioneer's strict quality controlled production system ensures that your results will be reproducible experiment after experiment.
•  Convenient: Just add template and primers and start your reaction. dNTPs, buffer and enzyme are provided
•  Stability: Stable at room temperature for a month and for 2 years in a -20°C freezer

Source: thermus aquatics
5' -> 3' exonuclease activity: Yes
3' -> 5' exonuclease activity: No
3' – A Overhang: Yes

Application

- Routine PCR
- Primer extension
- TA cloning
- Gene sequencing

Experimental data


Figure 1. Comparison of PCR amplification efficiency between AccuPower® Taq PCR PreMix from Bioneer and other suppliers' PCR master mix.

The cycling conditions for AccuPower® Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Target Gene : human myc.
Lane M : 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1 : 10 ng of human total cDNA
Lane 2 : 1 ng of human total cDNA
Lane 3 : 100 pg of human total cDNA
Lane 4 : 10 pg of human total cDNA
 

 
Figure 2. Comparison of PCR amplification efficiency between AccuPower® Taq PCR PreMix from Bioneer and other suppliers' PCR master mix.

The cycling conditions for AccuPower® Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Target Gene: IRGC (Immunity-related GTPase family, cinema)
Lane M : 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1 : 10 ng human genomic DNA
Lane 2 : 1 ng human genomic DNA
Lane 3 : 100 pg human genomic DNA
Lane 4 : 10 pg human genomic DNA
 

Figure 3. Comparison of PCR amplification of long targets between AccuPower® Taq PCR PreMix from Bioneer and other suppliers' PCR master mix.

The cycling conditions for AccuPower® Taq PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec, and 68°C for 8 min. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Lane M : 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1 : 3 kb fragment (human tumor protein p53 gene)
Lane 2 : 4 kb fragment (human beta globin region)
Lane 3 : 4.5 kb fragment (human DNA cross-link repair 1A gene)
Lane 4 : 8 kb fragment (human hemoglobin epsilon 1 gene)

• AccuPower® Taq PCR MasterMix

MSDS
• AccuPower® Taq PCR PreMix

Brochure
• AccuPower® PreMix series - 2010 Brochure

Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate