AccuPower DNA Ligation PreMix


Ready-to-use dna ligation master mix with T4 DNA Ligase



The AccuPower® DNA Ligation PreMix is a lyophilized master mix containing T4 DNA Ligase, ATP, reaction buffer, and patented stabilizer. This DNA ligation premix is conveniently aliquoted in strip-tubes for reactions; you need only add DNAs to be ligated and water. The reaction will work for DNA ligation for all applications: blunt cloning, sticky end cloning and TA cloning. The premix is stable up to four months at room temperature and for three years at -20°C.

Features and Benefits

  • Ready to use premix: Minimal set up time and handling required
  • Fast: Only 5 minutes for cohesive-end ligation and 10 minutes for blunt-end ligation at room temperature
  • Stable: Enzyme activity for up to four months at room temperature and for three years in the freezer

 

]]> Application

Cloning into vectors, library construction, TA cloning, linker ligation, and re-circlization of linear DNA


Figure 1. Stability test of AccuPower® DNA Ligation PreMix at room temperature.

Lane 1 – 12: Lambda DNA / Hind lll fragment (1 µg)
Lane 12 – 24: Lambda DNA / EcoR V fragment (1 µg)
Lane 2, 3, 14, 15: Ligation with AccuPower® Ligation PreMix stored at room temperature for 1 month
Lane 5, 6, 17, 18: Ligation with AccuPower® Ligation PreMix stored at room temperature for 2 months
Lane 8, 9, 20, 21: Ligation with AccuPower® Ligation PreMix stored at room temperature for 3 months
Lane 11, 12, 23, 24: Ligation with AccuPower® Ligation PreMix stored at room temperature for 4 months


Figure 2. DNA Ligation efficiency comparison between AccuPower® DNA Ligation PreMix and other competitors’ products.

Lane 1, 9: Intact Lambda DNA (1 µg)
Lane 2 – 8: Lambda DNA / Hind lll fragment (1 µg)
Lane 10 – 16: Lambda DNA / EcoR V fragment (1 µg)
Lane 3, 4, 11, 12: Ligation with AccuPower® Ligation PreMix
Lane 5, 13: Ligation with T4 DNA Ligase from company N
Lane 6, 14: Ligation with Quick Ligation Kit from company N
Lane 7, 15: Ligation with LigFast Rapid DNA Ligation System from company P
Lane 8, 16: Ligation with Ready-To-Go T4 DNA Ligase from company A

]]> Manual
• AccuPower® DNA Ligation PreMix


MSDS
• AccuPower® PCR Master Mixes


Brochure
• DNA Ligation 2010 Brochure


Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

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