AccuPrep® Gel Purification Kit


For the fragment DNA purification from the Agarose gel, Spin column type fragment DNA Extraction Kit – AccuPrep® Gel Purification Kit



AccuPrep® PCR Purification Kit is designed for the purification of up to 10 ug of DNA fragment from low-melting, TAE, TBE agarose gel fraction within 15 min. Provided Gel binding buffer can extracts the target DNA fragment from the agarose gel into the solution. The recovery yield exceeds 70~90%

Features and Benefits

Completely purify the fragment DNA from low-melting, TAE, TBE agarose gel fraction within 15 min.

Usable range is 100bp to 10kb with 70%-90% recovery.

Silica based DNA binding column with high binding efficiency.

 

Contents

Components K-3035 (200 reaction) K-3035-1 (50 reaction)
Buffer ① (Gel Binding buffer) 120 ml X 2 ea 30 ml X 2 ea
Buffer ② (Wahsing buffer) 25 ml X 2 ea 6 ml X 2 ea
Buffer ③ (Elution buffer) 15 ml X 1 ea 15 ml X 1 ea
DNA binding column tube 50 ea X 4 box 50 ea X 1 box
Manual 1 ea 1 ea
One Page Protocol 1 ea 1 ea

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Applications

Sub-cloning, Sequencing, Labeling, DNA concentration, etc

 

Procedure




Experimental Data


Fig. 1 Typical recovery

Comparison between AccuPrep® Gel Purification Kit and competitor.
This data shows the superior yield of AccuPrep® Gel PurificationKit as competing products.
B; AccuPrep® Gel Purification Kit (K-3035, K-3035-1), Q; competitor


Fig. 2 Recovery analysis of the purified fragment DNA

0.0 kb of plasmid DNA was purified from agarose gel after restriction enzyme digestion.
Lane M; Molecular weight marker, 100 bp Plus ladder (D-1035, Bioneer), Lane 100 ~ 50; control DNA(100% ~ 50%),
Lane B; AccuPrep® Gel Purification Kit (K-3035, K-3035-1)


 

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Manual

AccuPrep® Gel Purification Kit


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001:2008 - certificate


 

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