For plant tissue and seed samples; a Spin column based Genomic DNA Extraction Kit– AccuPrep® GMO DNA Extraction Kit
AccuPrep® GMO DNA Extraction Kit allows the extraction of DNA from agricultural products such as beans, corn, and rice or from processed foods such as bean sprouts, tofu and condensed milk, for the detection of GMOs. The kit includes all reagents required for extraction and in addition uses a column type extraction system to allow a more rapid, more convenient extraction of higher-quality DNA when compared to conventional methods. A high amount of high-purity DNA can be extracted; for example, 20 ~ 40 ug of DNA for 1 g of powdered bean, with an A260/A280 ratio of over 1.8
Features and Benefits
Highly purified and high yield genomic DNA can be extracted from various plant samples.
Silica based DNA binding column with high binding capacity.
Optimized plant lysis buffer for efficient lysis.
Contents
|
Components |
K-3031 (200 reactions) |
|
Lysis buffer (L) |
50 ml X 1 ea |
|
Binding buffer (B) |
50 ml X 1 ea |
|
Wahsing buffer 1 (W1) |
40 ml X 1 ea |
|
Washing buffer 2 (W2) |
20 ml X 2 ea |
|
Elution buffer (EL) |
25 ml X 1 ea |
|
Proteinase K, lyophilized |
25 mg X 2 ea |
|
DNA binding column tube |
100 ea X 1 pack |
|
2.0ml tubes for filtration |
100 ea X 1 pack |
|
1.5ml tubes for elution |
100 ea X 1 pack |
|
Manual |
1 ea |
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Specifications
|
Sample |
Amount |
Yield |
|
Soybean |
100 mg |
2 ~ 5 ug |
|
Maize |
100 mg |
1 ~ 4 ug |
|
Potato |
100 mg |
1 ~ 4 ug |
Applications
Gene cloning, PCR, Quantitative real time PCR, Southern blotting, SNP genotyping
Procedure
Experimental Data
Fig. 1 genomic DNAs from 0.1 g of soybean, tofu and maize
Lane M1: Molecular weight marker, λ DNA/EcoR l + Hind lll Markers (D-1070, Bioneer),
Lane M2: Molecular weight marker, 1 kb ladder (D-1040, Bioneer),
Lane 1: Soybean genomic DNALane 2: Tofu genomic DNALane 3: Maize genomic DNA,
* 100 ng of Intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested DNA (right)
Fig. 2 PCR results for GMO detection
Lane M: Molecular marker, 1 kb ladder (D-1040, Bioneer) Lane 1~5: PCR results for GMO detection
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Manual
• AccuPrep® GMO DNA Extraction Kit
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
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