For Whole blood, Animal tissue and Cultured cells; a Spin column based Genomic DNA Extraction Kit – AccuPrep® Genomic DNA Extraction Kit
AccuPrep® Genomic DNA Extraction Kit can rapidly and conveniently extract genomic DNA from blood, lymphocyte, buffy coat, tissue and cell cultures. This process does not require phenol/chloroform extraction, alcohol precipitation or other burdensome steps. The kit is based on spin column technology. Proteins and other contaminants which can inhibit enzyme reactions or PCR are eliminated through a series of short wash-and-spin steps. The isolated DNA is then ready to use in various applications.
Features and Benefits
Highly purified and high yield genomic DNA can be extracted from Whole blood, Animal tissue, Cultured cells.
Silica based DNA binding column with high binding efficiency.
Optimized Tissue lysis buffer for efficient lysis of the various tissue sample.
Contents
|
Components |
K-3032 (100 reactions) |
|
Tissue Lysis buffer (TL) |
25 ml X 1 ea |
|
Binding buffer (GC) |
25 ml X 1 ea |
|
Wahsing buffer 1 (W1) |
40 ml X 1 ea |
|
Washing buffer 2 (W2) |
20 ml X 2 ea |
|
Elution buffer (EL) |
30 ml X 1 ea |
|
Proteinase K, lyophilized |
25 mg X 2 ea |
|
DNA binding column tube |
100 ea X 1 pack |
|
2.0ml tubes for filtration |
100 ea X 1 pack |
|
1.5ml tubes for elution |
100 ea X 1 pack |
|
Manual |
1 ea |
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Specifications
|
Sample |
Amount |
Yield |
|
Whole blood |
200 ul |
3 - 6 ug |
|
Buffy coat |
200 ul |
15 - 20 ug |
|
Cultured cells |
104 ~ 108 cells |
15 - 20 ug |
|
Mammalian tissue |
25 ~ 50 mg |
10 - 15 ug |
Applications
Gene cloning, PCR, real time PCR, Southern blotting, SNP genotyping
Procedure
Experimental Data
Fig. 1 Gel electrophoresis of whole blood genomic DNA
Lane M1; Molecular weight marker, λ DNA/EcoR l + Hind lll Markers (Cat. No. D-1070, Bioneer),
Lane M2; Molecular weight marker, 1 kb ladder (Cat. No. D-1040, Bioneer),
Lane 1; whole blood genomic DNA of two individual mice,
Lane 2; whole blood genomic DNA of two individual rats,
Lane 3; whole blood genomic DNA of two persons.
* 100 ng of Intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested DNA (right)
Fig. 2 PCR with p53 primer set after genomic DNA purification
Lane M: Molecular marker, 100 bp ladder (D-1030, Bioneer), Lane 1~8: PCR product with p53 primer set
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Manual
• AccuPrep® Genomic DNA Extraction Kit
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
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