A novel Plasmid DNA Extraction Kit utilizing unique technology from Bioneer – AccuPrep® Nano-Plus Plasmid Maxi Extraction Kit : 45 min
The AccuPrep® Nano-Plus Plasmid Maxi Extraction Kit, extracts highly-purified plasmid DNA from cultured bacterial cells in in approximately 60 minutes. The overall principle utilizes a modified alkaline lysis protocol that is enhanced by Bioneer’s novel, patentented Nano-Technology method.
Features and Benefits
Principle is based on modified alkaline lysis protocol combined with a novel nano- particle technology
Suitable for both high-copy and low copy number plasmid DNA.
Highly purified and high purity plasmid DNA can be extracted from cultured E.coli cells within 10 min.
Endonuclease A denaturation buffer for the endA+ strains (Denaturation Buffer, Buffer D).
Silica based DNA binding column with high DNA binding efficiency.
Contents
|
Components |
K-3131 (25 reactions) |
K-3132 (10 reactions) |
|
Buffer ① (Resuspension buffer) |
240 ml X 1 ea |
96 ml X 1 ea |
|
Buffer ② (Lysis buffer) |
240 ml X 1 ea |
96 ml X 1 ea |
|
Buffer ③ (Neutralization buffer) |
240 ml X 1 ea |
96 ml X 1 ea |
|
Buffer ④ (Washing buffer) |
80 ml X 3 ea |
30 ml X 3 ea |
|
Buffer ⑤ (Elution buffer) |
50 ml X 1 ea |
20 ml X 1 ea |
|
RNase A, lyophilized |
24 mg X 1 ea |
9.6 mg X 1 ea |
|
Clearing Syringe Filter (30ml) |
25 ea X 1 pack |
10 ea X 1 pack |
|
DNA Binding Filter Tubes |
25 ea X 1 pack |
10 ea X 1 pack |
|
Manual |
1 ea |
1 ea |
|
One Page Protocol |
1 ea |
1 ea |
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Specifications
|
Starting culture volume |
100 ml ~ |
|
Column binding capacity |
> 500 ug |
|
Elution volume |
1 ml |
|
Expected DNA yield |
Up to 300 - 500 ug |
|
Preparation time |
< 60 min |
Applications
Sub-cloning, Sequencing, Transformation, Trasnfection, In-vitro transcription/ translation
Procedure
Experimental Data
Fig. 1 Reduced Total Prep. Time
The nano-particles which included in the Buffer ①(Resuspension buffer) can make a complex together with insoluble protein aggregate, cell debris and chromosomal DNA in the solution. And the complex can be separated from the solution with simple centrifuge step(10 min.) to obtain a cleared lysate which contains target plasmid DNA
B; AccuPrep® Nano-Plus Plasmid Maxi Extraction Kit, Q, P; Competitors
Fig. 2 Improved Prep. Yield
Plasmid DNA purified with the AccuPrep® Nano-Plus Plasmid Mini Extraction Kit AccuPrep® Nano-Plus Mini Kit provides high yields of plasmid DNA.
B; AccuPrep® Nano-Plus Plasmid Maxi Extraction Kit (K-3131, K-3132), P, Q; competitors
Fig. 3 Restriction enzyme digestion test
Electrophoresis results with extracted plasmid DNA after restriction enzyme digestion with various restriction enzymes. pBS; pBluescript SK(+) with XbaI, pBI121; pBI121 with PstI, Lane M; Molecular Marker (λ-DNA/ HindIII+EcoRI, Cat. No. D-1070, Bioneer), Lane B; AccuPrep® Nano-Plus Plasmid Maxi ExtractionKit, Lane Q, P; competitors
Electrophoresis results with extracted plasmid DNA after restriction enzyme digestion with various restriction enzymes. Left; pBluescriptSK(+), Right; pBI121, Lane M; Molecular Marker (Cat. No. D-1040, Bioneer)
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Manual
• AccuPrep® Nano-Plus Plasmid Extraction Kit (Mini/Midi/Maxi)
MSDS
• AccuPrep
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
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