Enzyme Properties
5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: Yes
3’ – A Overhang: No
Fragment Size: < 20 kb
Application
Gene synthesis
PCR or Primer extension requested High fidelity
Blunt-end PCR Cloning or mutagenesis requested high fidelity
Reagents Supplied
10 x Reaction Buffer: MgSO4, Tris-HCl, KCl, (NH4)2SO4,Acetylated BSA pH 8.8
dNTP Mix: 2.5 mM of each dNTP(option)
Concentration
2.5 U/μl
Store Temperature
-20°C
Unit Definition
One unit is defined at the amount of enzyme that will incorporate 10 nmole of dNTP into acid-insoluble material in 30 minutes at 72°C.
Activity Test
Amplication of limiting amounts of template DNA: comparison of Lambda DNA (A), Bacterial genomic DNA (B), Human genomic DNA (C).
* Lambda DNA was amplified using 2.5 units of enzyme in 50 uL reaction volume.
* Bactierial DNA was amplified using 2.5 units of enzyme in 50 uL reaction volume.
* Human DNA was amplified using 2.5 units of enzyme in 50 uL reaction volume.
Amplification of fragments ranging from 500 bp to 5 kb from template Lambda DNA 100 pg with 2.5 units of Pfu DNA Polymerase.
Notice to Purchaser
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES
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Manual
• Pfu DNA Polymerase
MSDS
• Pfu DNA Polymerase
• Pfu DP-dilution buffer
• Pfu DP-reaction buffer
Brochure
• Enzymes 2010 Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
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