Taq DNA Polymerase


Versatile DNA polymerase for everyday routine PCR



Taq DNA Polymerase is a thermostable DNA polymerase that catalyzes the polymerization of nucleotides into duplex DNA in the 5' -> 3' direction. Bioneer's Taq DNA Polymerase is isolated from recombinant E.coli strain containing the DNA polymerase gene from Thermus aquaticus YT1. It exhibits its highest activity at pH 9.0 and 72°C.

Features and Benefits

  • Improved yield & sensitivity:  Perform high yield and high sensitivity PCR using Bioneer Taq DNA polymerase.
  • Versatility: Use for a wide range of DNA amplifications including Real-Time PCR using TaqMan probe or SYBR Green.
  • Robust performance:  Optimized reaction buffer enhances PCR performance.

]]> Enzyme Properties

Concentration: 5 U/ul
5' to 3' exonuclease activity: Yes
3' to 5' exonuclease activity: No
3’ – A Overhang: Yes
Nuclease contamination: No
Extension rate: 3-10 kb/min depending on template complexity
Fragment Size: Up to 10 kb
 

Application

- Routine PCR
- SYBR-Green-based qPCR
- Dual-labeled probe based qPCR
- Primer extension
- TA cloning
- Gene sequencing
- Gene expression profiling
- Microbial & viral pathogen detection


Kit Contents

Cat. No. Taq DNA Polymerase 10 x Rxn Buffer Dilution Buffer dNTP Mixture MgCl2 Sol.
E-2011 500 units 1 ml (with MgCl2) 1 ml 1 ml -
E-2011-1 500 units 1 ml (without MgCl2) 1 ml 1 ml 1 ml
E-2011-2 500 units 1 ml (with MgCl2) 1 ml - -
E-2011-3 500 units 1 ml (without MgCl2) 1 ml - 1 ml

- 10 x Reaction Buffer without MgCl2: 100 mM Tris-HCl, 400 mM KCl, pH 9.0
- 10 x Reaction Buffer with MgCl2: 100 mM Tris-HCl, 400 mM KCl, 15 mM MgCl2, pH 9.0
- Dilution Buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 50 % glycerol, pH 8.0
- dNTP Mixture: 10 mM (2.5 mM each dNTP)
- MgCl2 Solution: 20 mM


Storage Conditions

Taq DNA Polymerase, including buffers and reagents, should be stored immediately upon receipt at –20°C. If stored in the recommended temperature, this product will be stable until the expiration date printed out on the label.

 

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C.

 

Experimental data

Figure 1. Taq DNA Polymerase sensitivity test

M: 1 kb DNA Ladder (Bioneer, Cat. No. D-1040,)
Lane 1: 100 ng Template DNA
Lane 2: 10 ng Template DNA
Lane 3: 1 ng Template DNA
Lane 4: 100 pg Template DNA
Lane 5: 10 pg Template DNA
Lane 6: 1 pg Template DNA
Lane 7: 100 fg Template DNA

Figure 2. Taq DNA polymerase serial dilution test

M: 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
Lane 1: 5 U of Taq DNA polymerase
Lane 2: 1 U of Taq DNA polymerase
Lane 3: 0.5 U of Taq DNA polymerase
Lane 4: 0.33 U of Taq DNA polymerase
Lane 5: 0.25 U of Taq DNA polymerase

Figure 3. Taq DNA Polymerase long Kb DNA

M : 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
Lane 1 : 5 kb PCR product
Lane 2 : 6 kb PCR product
Lane 3 : 7 kb PCR product
Lane 4 : 8 kb PCR product

 



 

 

 

 

 

 

 


]]> Manual
• Taq DNA Polymerase


MSDS
• Taq DNA Polymerase


Brochure
• Taq DNA Polymerase Brochure


Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

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