Protein Expression and Purification


A line of instrumentation and kits designed for synthesis and purification of high-quality protein – rapidly and easily

With the latest technology and years of experience designing and manufacturing products for protein synthesis and purification, Bioneer is pleased to showcase our Accu- and Exi- series products for protein science. These products are designed to simplify your protein work in a cost-effective way.
 

At the cutting edge, Bioneer has recently launched the world's first automated protein synthesis/purification and nucleic acid purification system: ExiProgen.
ExiProgen™ makes protein synthesis and purification as easy as a thermal cycler makes PCR. In order to achieve this ease of use, we have developed the ExiProgen™ EC1 Protein Synthesis Kit for cell-free in vitro protein expression and purification. The technology is based on the AccuRapid™ Cell-Free Protein Expression Kit and Ni-NTA magnetic beads used in our AccuPrep® His-tagged Protein Purification Kit. The ExiProgen™ EC1 Protein Synthesis kit is able to express and purify up to 16 unique proteins in parallel in less than 6 hours.

BIONEER's line of protein-related products not only perform standard protein expression and purification, but also provide an automated system on which to perform the tasks, providing the optimal solution needed to enter the Bio 2.0 era.

All protein-related products are mass produced under a one-batch system governed by ISO 9001 quality standards for world-class results and utmost reproducibility.

Features and Benefits

ExiProgen™/ExiProgen™ EC1 Protein Synthesis Kit

Fully automatic: DNA-in-Protein-out:
ExiProgen™ EC1 Kit and AccuRapid™ Cell-Free Protein Expression Kit require only the addition of template DNA which can be in to form of an expression vector such as pBIVT or a PCR product as can be made with the ExiProgen ProXpress PCR Template Kit.

Parallel Process
Obtain from 1 to 16 different proteins in a single run.

High performance
All systems allows for synthesis of proteins that are toxic to in vivo expression systems.

Reproducibility
The only hands-on step is the addition of template DNA, maximizing reproducibility.

Fast procedure
The in vitro (cell-free) protein synthesis system does not require time-consuming cell culture processes.
 

AccuRapid™ Cell-Free Protein Expression Kit/ ExiPrep™ & AccuPrep® His-tagged Protein Purification Kit

High-speed
AccuRapid™ Cell-Free Protein Expression Kit reactions are carried out in just 3 hours. His-Tag purification can be accomplished in 30 minutes!

Simplicity
The AccuRapid™ Cell-Free Protein Expression Kit contains all necessary components for transcription and translation. Simply add template and incubate!

Flexible
The AccuRapid™ Cell-Free Protein Expression Kit can synthesize proteins from various DNA templates.

SSMB (Spherical Shape Magnetic Beads) for His-Tag purification
SSMB beads have increased surface area by virtue of their size and eliminate the carryover problems of rough surfaced magnetic particles.

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  Accu- series for manual method Exi- series for automated method
Protein Expression AccuRapid™ Cell-Free Protein Expression Kit ExiProgen™ EC1 Protein Synthesis Kit
Protein Purification AccuPrep® His-tagged Protein Purification Kit ExiProgen™ His-tagged Protein Purification Kit


1. Accu- series: Accu- series products are designed for manual workflows.

AccuRapid™ Cell-Free Protein Expression Kit
This product is an adaptation of a cell-free protein expression system.
It includes all necessary components for successful in vitro protein expression. A total of twenty-four (24) 45uL volume reactions are possible with this product.

AccuPrep® is-tagged Protein Purification Kit
This kit is designed for the convenient purification of Histidine-tagged proteins from E. coli lysate.
Purification is based on Ni-NTA magnetic beads for magnetic or centrifugal purification.

 

2. Exi- series: ExiProgen series products are designed for Bioneer's automated systems (ExiProgen™).

ExiProgen™ EC1 Protein Synthesis kit
This product is designed to yield world-class protein synthesis performance when combined with the world’s first automated protein synthesis and nucleic acid purification system ExiProgen™. Up to 16 different proteins can be expressed and purified simultaneously within 6 hours of adding template DNA, and automation means that the results will be reproducible run after run.

ExiProgen™ His-tagged Protein Purification Kit
This product is designed for the purification of up to 16 His-tagged proteins simultaneously within 30 minutes with excellent reproducibility from automation

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