Master mix for DNA amplification requiring high fidelity capability
AccuPower® Pfu PCR PreMix is a lyophilized mixture of Pfu DNA polymerase, dNTPs and reaction buffer in a convenient premix format. Simply add template, primers and water and mix – AccuPower® offers easy set-up for every PCR application. Bioneer's patented stabilizer maintains the activity of the PreMix for over a month when stored at room temperature (25°C) and for over 2 years in the freezer (-20°C).
Features and Benefits
High fidelity : AccuPower® Pfu PCR PreMix is a high fidelity (error rate = 1.9x10-6)enzyme that will reduce errors during DNA amplification.
High purity : AccuPower® Pfu PCR PreMix is a recombinant enzyme that eliminates smearing and unwanted background found in native Pfu enzymes.
Thermostability : Pfu has an optimal activity that is higher than most other thermostable polymerases, and exhibits low activity at temperatures below 50°C. This results in higher specificity for your PCR reactions.
Stability : To preserve the activity during storage, the PCR PreMix has been freeze-dried with a special stabilizer. Bioneer's unique stabilizing agent maintains the DNA polymerase activity for over 2 years in the freezer (-20°C).
Simplicity : Almost all of the reaction chemicals required for PCR, such as thermostable DNA polymerase and dNTPs, are premixed into a single unit. The user simply adds template DNA, primers, and ddH2O to start the reaction. A tracking dye and polymers for gel electrophoresis are also included in the mixture so there is no need to add any sample loading buffer.
Reproducibility : AccuPower® products are manufactured under strict ISO 9001 quality control conditions to ensure reproducible PCR performance, experiment after experiment.
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Specifications
5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: Yes
3' – A Overhang: No
Fragment Size: Up to 10 kb
Application
- Gene synthesis
- Gene cloning
- Primer extension
- Site directed mutagenesis
- High-fidelity PCR
Storage Temperature
-20°C
Experimental Data
Figure 1. AccuPower® Pfu PCR PreMix Sensitivity test
AccuPower® Pfu PCR PreMix was tested with serial dilutions of Lambda DNA template. (The PCR product size is 1 kb) The cycling conditions were 95°C for 5min, 30 cycles of 95°C for 30 sec, 55°C for 30 sec, and 72°C for 1 min, and 72°C for 5 min for final extension
M: 100 bp DNA Ladder (Cat. No. D-1030)
Line 1: 10ng Line 5: 1pg,
Line 2: 1ng Line 6: 100 fg,
Line 3: 100pg Line 7: 10 fg,
Line 4: 10pg Line 8: No Template
Figure 2. AccuPower® Pfu PCR PreMix sensitivity test with serial dilutions of Human DNA template.
The cycling conditions were 95°C for 5min, 30 cycles of 95°C for 30 sec, 55°C for 30 sec, and 72°C for 1 min, and 72°C for 5 min for final extension
M: 100 bp DNA Ladder (Cat. No. D-1030)
Line 1: 100ng Line 4: 100pg
Line 2: 10ng Line 5: 10 pg
Line 3: 1ng Line 6: No Template
Figure 3. Amplification of Human genomic DNA fragments from 1 kb to 10 kb using AccuPower® Pfu PCR PreMix.
M: 1 kb DNA Ladder (Cat. No. D-1040)
Line 1: 1 kb fragment Line 6: 6 kb fragment
Line 2: 2 kb fragment Line 7: 7 kb fragment
Line 3: 3 kb fragment Line 8: 8 kb fragment
Line 4: 4 kb fragment Line 9: 9 kb fragment
Line 5: 5 kb fragment Line 10: 10 kb fragment
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Manual
• AccuPower® Pfu PCR PreMix
MSDS
• MSDS_AccuPower® Pfu PCR PreMix
Brochure
• AccuPower® Pfu PCR PreMix - 2011 Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
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