Specifications
Source: thermus aquatics
5' -> 3' exonuclease activity: Yes
3' -> 5' exonuclease activity: No
3' – A Overhang: Yes
Application
- Routine PCR
- Primer extension
- TA cloning
- Gene sequencing
Experimental data
Figure 1. Comparison of PCR amplification efficiency between AccuPower® Taq PCR PreMix from Bioneer and other suppliers' PCR master mix.
The cycling conditions for AccuPower® Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Target Gene : human myc.
Lane M : 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1 : 10 ng of human total cDNA
Lane 2 : 1 ng of human total cDNA
Lane 3 : 100 pg of human total cDNA
Lane 4 : 10 pg of human total cDNA
Figure 2. Comparison of PCR amplification efficiency between AccuPower® Taq PCR PreMix from Bioneer and other suppliers' PCR master mix.
The cycling conditions for AccuPower® Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Target Gene: IRGC (Immunity-related GTPase family, cinema)
Lane M : 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1 : 10 ng human genomic DNA
Lane 2 : 1 ng human genomic DNA
Lane 3 : 100 pg human genomic DNA
Lane 4 : 10 pg human genomic DNA
Figure 3. Comparison of PCR amplification of long targets between AccuPower® Taq PCR PreMix from Bioneer and other suppliers' PCR master mix.
The cycling conditions for AccuPower® Taq PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec, and 68°C for 8 min. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Lane M : 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1 : 3 kb fragment (human tumor protein p53 gene)
Lane 2 : 4 kb fragment (human beta globin region)
Lane 3 : 4.5 kb fragment (human DNA cross-link repair 1A gene)
Lane 4 : 8 kb fragment (human hemoglobin epsilon 1 gene)
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Manual
• AccuPower® Taq PCR PreMix
• AccuPower® Taq PCR MasterMix
MSDS
• AccuPower® Taq PCR PreMix
Brochure
• AccuPower® PreMix series - 2010 Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
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