For various clinical samples, Automatic viral DNA/ RNA Extraction Kit – ExiProgen™ Viral DNA/ RNA Kit
ExiProgen™ Viral DNA/ RNA Kit
ExiProgen™ Viral DNA/ RNA Kit is a product designed to extract high-purity viral DNA/ RNA from c serum, plasma, CSF, saliva, swab and cell free body fluid samples using the automated nucleic acid extraction instrument ExiProgen™ 16 Plus. The binding buffer and silica magnetic particles will bind viral DNA/ RNA to the surface of the beads. The reaction containers are then subject to a magnetic field, forcing the viral DNA/ RNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially during in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the viral DNA/ RNA-bound silica magnetic particles remain bound by the magnetic field. DNase RNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified viral DNA/ RNA.
Features and Benefits
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Reproducibility & Reliability
ExiProgen™ Viral DNA/ RNA Kit functions through a buffer cartridge system containing buffers optimized for the extraction of total RNA from serum, plasma, CSF, saliva, swab and cell free body fluid samples. Nucleic acid extractions occur with the buffer cartridges through the automated nucleic acid extraction instrument, allowing for reproducible performance. Also, by preventing human errors during the workflow, the results are always dependable.
The product is produced under ISO 9001 quality standards by an automated production facility to provide you with high-quality performance.
The buffer composition within the ExiProgen™ Viral DNA/ RNA Kit was developed to extract high purity and high yield total RNA from serum, plasma, CSF, saliva, swab and cell free body fluid samples. Also, the extraction protocols contained within the automated nucleic acid extraction instruments ExiProgen™ 16 Plus is optimized for maximum performance. Anyony can extract total RNA of high purity and yield from cultured cell samples by using the pre-programmed and optimized protocols in ExiProgen™ 16 Plus.
Contents
|
Components |
Quantity |
Note |
|
Buffer Cartridge ① |
6 ea |
|
|
Buffer Cartridge ② |
6 ea |
|
|
Disposable filter tip |
3 pack |
32 ea/pack |
|
Elution tube |
1 pack |
12 ea/pack |
|
Manual |
1 ea |
|
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Applications
PCR, RT-PCR, Quantitative real time PCR, Quantitative real time RT-PCR
Procedure
Experimental Data
Fig. 1 Real-time PCR result(HBV) of viral DNA extracted from serum
Real-time PCR results using an AccuPower® HBV Quantitative PCR Kit(HBV-1111, Bioneer) and run on Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060, Bioneer) with extracted viral DNA.
Fig. 2 Real-time RT-PCR result(HCV) of viral RNA extracted from serum
Real-time RT-PCR results using an AccuPower® HCV Quantitative PCR Kit(HCV-1111, Bioneer) and run on Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060, Bioneer) with extracted viral RNA.
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Application Note
• ExiProgen™ Viral DNA/RNA Kit
Manual
• ExiProgen™ Viral DNA/RNA Kit
MSDS
• ExiProgen™ Viral DNA/RNA Kit
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
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