• Mutagenesis


Deletion, insertion and point mutations. 100% sequence guarante

The fastest and most cost effective way to construct a mutant gene for protein structure and function research or to increase enzyme function is to use our Mutagenesis Service combined with our Gene Synthesis Service. Site-directed mutation such as Point Mutation as well as deletion, insertion mutation services are available. For unsurpassed quality and value pricing, partner with Bioneer today!


Features and Benefits

  • 100% Sequence Guarantee: All point mutation as well as deletion mutation products are 100% confirmed by sequencing
  • Codon Optimization – Free of charge!: Complimentary codon optimization to enhance protein expression and function
  • Value Pricing: The best value for your research dollar

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Applications


Antibody Construction
Antibodies targeted toward specific diseases or targets can be codon optimized for maximum expression in the host organism. Also, an antibody library can be constructed to screen for the most efficient antibody variant.
 

Organism Production Optimization
Optimize expression of genes related to resource production to maximize industrial biological production efficiency.
 

Gene Construction
Get difficult-to-obtain DNA sequences without a template and upgrade the quality of your research by constructing hypothetical genes.
 

Protein Modification
Codon optimization can increase protein expression efficiency, and a mutant library derived from this process can yield proteins with increased function. Optimizations include secondary structure removal, and repeat reduction as well as organism optimizations.
 

Schematics of Gene Synthesis Process

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MSDS

Gene Synthesis Service


Brochure

Gene Synthesis Service


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001:2008 - certificate


 

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Download  Gene Synthesis Order Form

Mutagenesis Service
Accepted Materials Plasmid, Cultured E. coli cell
Price $200.00/point mutation (Stand-alone order/ ~ 1kb insert)
Additional Costs With cultured cells $ 50.00
$50.00/500bp (Orders of inserts of over 1kb)
Service Period ~ 1 kb 10 ~ 15 working days
1kb ~ 3kb 15 ~ 25 working days
3kb ~ 5kb 25 ~ 40 working days
5kb ~ Inquire
Additional Service Produce high yield of plasmid DNA ($100.00/100ug)
Proceed with Gene synthesis ($100.00 discount)


* Additional charges will apply for gene segments containing complexities such as high or low GC content, repeat sequences or homopolymeric runs. If any of your sequence is found to contain the above listed complexities, you will be contacted by our Gene Synthesis Specialist.
* When choosing the subcloning service with a commercial vector, a vector purchase cost will be billed separately.
* Synthesized genes will be sequenced. Only 100% sequence identity products will be shipped.
* 50% charge of service bill for cancellation in 5days.
80% charge of service bill for cancellation after 5days.
Free of charge for cancellation after guarantee duration(Guarantee duration: gene synthesis period except delivery)


Quotations and Ordering

1. Download the ordering form(s) from the link on top.
2. Fill out the form and email the form to: contact@bioneer.com.au
3. We will review the order and send you service information (final quote and service period) via email.
4. If you decide to go ahead with the service, please confirm the service by an email.
5. The service will be initiated when we send out the service start alert email.
(For the Cloning and Point mutation services, the service begins when we receive the raw material from you.)
* Please fill in the personal information columns (contact information).
We need it to fine-tune the service and expedite the order.

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